CRISPRi Screen Discovers Mitochondrial Distress Signal
The function of mitochondria, the “powerhouse of the cell,” can be compromised in aging and disease. Dysfunctional mitochondria trigger a global cellular stress response, but how human mitochondria signal stress to the rest of the cell was unknown.
In a project led by postdoc Xiaoyan Guo in the Kampmann lab, a CRISPRi-based genetic screen uncovered the molecular mechanism by which mitochondrial dysfunction is relayed to the rest of the cell. The mitochondrial protease OMA1 cleaves a previously little characterized protein, DELE1. Cleaved DELE1 activates the kinase, HRI, triggering the so-called integrated stress response.
This newly... Read more ...